Generator

Part:BBa_K1179075:Design

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-18)


TRE-t_eYFP-4x503


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1233
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1209
    Illegal PstI site found at 590
    Illegal PstI site found at 1117
    Illegal PstI site found at 1140
    Illegal PstI site found at 1163
    Illegal PstI site found at 1186
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1233
    Illegal SpeI site found at 1209
    Illegal PstI site found at 590
    Illegal PstI site found at 1117
    Illegal PstI site found at 1140
    Illegal PstI site found at 1163
    Illegal PstI site found at 1186
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1233
    Illegal BamHI site found at 353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1233
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1209
    Illegal PstI site found at 590
    Illegal PstI site found at 1117
    Illegal PstI site found at 1140
    Illegal PstI site found at 1163
    Illegal PstI site found at 1186
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 1233
    Illegal XbaI site found at 30
    Illegal SpeI site found at 1209
    Illegal PstI site found at 590
    Illegal PstI site found at 1117
    Illegal PstI site found at 1140
    Illegal PstI site found at 1163
    Illegal PstI site found at 1186
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There will still be low yellow fluorescence background expression in the presence of miRNA503. It is recommended that people interested in using this part, test for this small amount of background expression before performing experiments with this part.

Source

Constructed by performing a gateway reaction with entry vectors TRE-tight and eYFP-4x503ts. See components for more information

References